Fig. 6

Production of reactive oxygen species by blood and sputum neutrophils in PCD and bronchiectasis. Peripheral blood neutrophils from patients with PCD (PCD blood, n = 6–10) and non-PCD, non-CF bronchiectasis (BE blood, n = 4–5) and purified sputum cells from patients with PCD (PCD sputum, n = 6–10) and non-PCD, non-CF bronchiectasis (BE sputum, n = 4–5) were primed with TNF-α and exposed to PMA or bacterial stimuli. By detection of luminol chemiluminescence (expressed as relative light units, RLU), the production of reactive oxygen species (ROS) was measured. A A representative graph showing the pattern of ROS production by blood and sputum neutrophils over time in absence of stimulation. ROS production is represented in RLU. B A representative graph showing the pattern of ROS production by blood and sputum neutrophils stimulated with LPS. C Spontaneous release of ROS by unstimulated cells. D–G ROS release in response to stimulation with D PMA, E LPS, F PGN or G fMLF is represented here as fold change relative to the unstimulated condition