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Fig. 5 | Respiratory Research

Fig. 5

From: Sputum from patients with primary ciliary dyskinesia contains high numbers of dysfunctional neutrophils and inhibits efferocytosis

Fig. 5

Levels of polymerized actin in peripheral blood and sputum neutrophils in PCD and bronchiectasis. Purified blood neutrophils and sputum cells from patients with A, B. PCD (n = 4–5) and C, D non-PCD, non-CF bronchiectasis (n = 3) were exposed for 30 s to a chemotactic stimulus, whereupon the cells were fixed and stained with AF555-phalloidin. Using flow cytometry, the total F-actin content per cell was determined in neutrophils (defined as CD16 + CD66b + cells). Data are shown as A, C median fluorescence intensities (MFI) of AF555-phalloidin in unstimulated cells or as B, D the fold increase in F-actin content (MFI) in stimulated cells, relative to the MFI of unstimulated cells

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Respiratory Research

ISSN: 1465-993X

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