Fig. 2
Cell viability and cell composition of sputum cells in PCD and bronchiectasis. A Purified peripheral blood neutrophils and purified sputum cells from patients with PCD (black circles, n = 13) and non-CF, non-PCD bronchiectasis (open circles, n = 5) were stained with live/dead dye, and the percentage of live cells was determined by flow cytometry. B Purified sputum cells from patients with PCD (black circles, n = 5) and non-CF, non-PCD bronchiectasis (open circles, n = 3) were labeled with antibodies directed against lineage markers (CD16 and CD66b for neutrophils; CD14 and HLA-DR for macrophages; CD4 for helper T lymphocytes; CD8 for cytotoxic T lymphocytes; CD19 for B cells; CD56 for NK cells). Using flow cytometry, the proportion of each leukocyte subpopulation within live cells in the sputum was determined. C Association between total number of cells isolated from the sputum samples of patients with PCD (black circles, n = 17) and non-CF, non-PCD bronchiectasis (open circles, n = 5) and the FEV1% pred value, p = 0.6238. D Association between the percentage of live cells observed in sputum from patients with PCD (black circles, n = 12) and non-CF, non-PCD bronchiectasis (open circles, n = 5) (as determined by the cells being negative for live/dead staining) and the FEV1% pred value, p = 0.5920. E Association between the percentage of neutrophils in the sputum of patients with PCD (black circles, n = 12) and non-CF, non-PCD bronchiectasis (open circles, n = 5) (defined as the proportion of live cells that was positive for both CD16 and CD66b) and the FEV1% pred value, p = 0.0615