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Fig. 3 | Respiratory Research

Fig. 3

From: FGL1 regulates acquired resistance to Gefitinib by inhibiting apoptosis in non-small cell lung cancer

Fig. 3

Knockdown of FGL1 increases the sensitivity of PC9/GR cells to gefitinib in vitro (x ± s, n = 3). a Relative expression of FGL1 protein in PC9/GR cells after FGL1 interference as measured by western blotting. b Relative expression of FGL1 mRNA in PC9/GR cells after FGL1 knockdown as measured by RT-qPCR. c Proliferation of PC9/GR cells inhibited by gefitinib after FGL1 knockdown as measured by CCK-8. d Comparison of IC50 values of gefitinib in PC9/GR cells after FGL1 knockdown. e Colony-forming potential was assessed in PC9/GR cells. f Apoptotic rate of PC9/GR cells as evaluated by flow cytometry. Control: blank control group; NC-siRNA: mice transfected with NC-siRNA; Gefitinib: mice treated with gefitinib alone; FGL1-siRNA: mice treated with FGL1-siRNA-transfected cells; gefitinib+FGL1-siRNA: mice transfected with FGL1-siRNA and treated with gefitinib; *P < 0.05 compared with control group; **P < 0.01 compared with control group; #P < 0.05 compared with gefitinib group; (P < 0.05 compared with gefitinib group; â–³P < 0.01 compared with FGL1-siRNA group; &P < 0.01 compared with PC9 cells

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Respiratory Research

ISSN: 1465-993X

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