Fig. 2

SDF-1 Knockdown Attenuates MSC Regenerative Potential On Lung Alveolarization. a Hematoxylin and eosin stained lung sections demonstrating improved alveolar structure in hyperoxia-exposed pups treated with IT MSC-NS control. This improvement was significantly decreased following SDF-1 knockdown in MSC (MSC-SDF KD). Original magnification X 200. Scale bars are 100 μm. Morphometric analyses revealed that while IT MSC-NS control reduced the (b) mean linear intercept and (c) alveolar volume fraction in hyperoxic animals, administration of IT MSC SDF KD reversed these beneficial effects. (*P < 0.05; normoxia vs. hyperoxia-placebo, ** P < 0.05; hyperoxia-placebo vs hyperoxia MSC-NS control, † P < 0.05; hyperoxia MSC-NS control vs. hyperoxia MSC-SDF KD; N = 5–7/group). White bars are room air (RA) and black bars are hyperoxia