Fig. 2

IPF fibroblast derived supernatants elevate integrin α5 (ITGA5) levels. IPF/normal supernatants were added to fibroblast cells seeded on plastic or fibronectin (FN) (10 μg/ml) coated plates. Following culture, cells were harvested for protein extraction and their adhesion to the FN was tested. Levels of ITGA5 were analyzed by western blotting (n = 13) (a). Cell adhesion to FN was quantified by microscopic cell count (n = 5) (b). Results were normalized to control (N-SN) and considered significant * if p < 0.05 (n ≥ 4), error bar represents the standard error. (c) Representative photomicrographs of increased FN attachment of fibroblast cells cultured with IPF-SN (right) following 24 h