Fig. 3
From: Role of P2X7R in the development and progression of pulmonary hypertension
Activation of the P2X7R and inflammasome in PH rats. Animals were treated with P/MCT, P/MCT plus early and continuous A740003 (P/MCT + CA), or P/MCT plus delayed A740003 (P/MCT + DA) as described in the METHODS. The levels of P2X7R (70 kDa), NLRP3 (118 kDa) (a) procaspase-1 (Pro-Casp1, 40 kDa), active caspase-1 (Casp1, 20 kDa) (b) and mature IL-1β (20 kDa) (c) were measured in total lung homogenates from PH rats 4 weeks after MCT exposure using western blot. Quantification of protein expression is shown, respectively (c–f, h); GAPDH was used as a loading control (37 kDa). Data are shown as the mean ± SD, n = 6, *p < 0.05 compared with the sham group; †p < 0.05 compared with the P/MCT vehicle group