Fig. 3

Impact of HDAC2 knockdown on NF-κB activation in primary skeletal muscle cells. Effect of HDAC2 RNA interference on HDAC2 protein (a), NF-κB activity (b), NF-κB -p65 acetylation at lysine³¹⁰ (c), basal TNF-α production (d) at 24 h after transfection, cell number determined by MTT assay (e) and cell apoptosis evaluated with caspase 3 activity assay (f) in primary skeletal muscle cells. IKK inhibitor (IKKi), SC514, was given 12 h after transfection. *: p < 0.05, **: p < 0.01. Abbreviations: nuclear factor-kappa B (NF-κB), histone deacetylase (HDAC), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), no transfection (NT), negative control short interfering RNA (SiNeg), short interfering RNA of HDAC2 (SiHD2); IκB kinase (IKK), optical density (OD), acetylated p65 (Ac-p65), methylthiazol tetrazolium (MTT:)