Fig. 1

Example of the isolation of a pulmonary NEB from a GAD67-GFP mouse lung cryosection using LMD (see Methods). a GFP-fluorescent NEB (arrowhead) located in the epithelium of an intrapulmonary airway. b Image taken after LMD. The area circled by the red line shows the region of interest that was selected to be cut by the laser. c Isolated GFP-fluorescent NEB, captured in the cap of an Eppendorf tube and ready for consecutive pooling and RNA isolation. Note that even after very mild fixation, to optimally preserve RNA quality, and without cover glass, NEBs appear to be unambiguously detectable in the LMD microscope (Leica LMD7000; 20x objective). L: airway lumen, E: airway epithelium