Fig. 4

IL10 recapitulates the EPFR-induced exacerbation of influenza morbidity in Air-exposed mice. a Schematic for rIL10 administration and i.n. influenza infection and assessment of percentage weight change and peak pulmonary viral load at four dpi. b Average percent body weight change ((value-baseline/baseline)*100) over a period of 11 dpi in Air-exposed mice treated with rIL10 and subsequently (2 h-post treatment) infected with influenza virus. c Pulmonary viral load in Air/Flu and rIL10/Flu mice at 4 dpi. Limit of detection is indicated by the dotted line. d Immunohistochemical staining for influenza virus in the lungs of Air/Flu, DCB/Flu, and rIL10/Flu mice at 4 dpi. Data were plotted as means ± SEM; *p < 0.05 and **p < 0.01; (b) Multiple comparisons t tests with Holm-Sidak correction for multiple comparisons and (c) Student’s t-test