Fig. 4
From: Induction of pulmonary antibodies against oxidized lipids in mice exposed to cigarette smoke
Anti-OxPL antibodies increase smoke particle uptake by pulmonary macrophages when delivered during smoking cessation. a 6–8 week old female C57BL/6 mice were exposed to room air (RA) or cigarette smoke (CS) for 1 h, twice a day, 5 days a week for 8 weeks. They were then subjected to 2 days of complete smoking cessation and, on the morning of the following 3 consecutive days, received phosphate-buffered solution (35 μl; PBS), a mouse IgM isotype control (20 μg in 35 μl; ISO), or a mouse monoclonal antibody against oxidized phosphadytilcholine (20 μg in 35 μl; clone E06) intranasally. b Black smoke particles were counted in pulmonary macrophages and expressed as the absolute number of macrophages with no (white), 1–4 (grey) or >5 (black) intracellular particles. c A representative cytospin from cigarette smoke-exposed mice that received the E06 antibody during cessation