Fig. 3
From: Induction of pulmonary antibodies against oxidized lipids in mice exposed to cigarette smoke
Anti-OxPL antibodies increase lipid and smoke particle uptake by pulmonary macrophages. Female 6–8 week old C57BL/6 mice were exposed to room air (RA) or cigarette smoke (CS) for 1 h, twice a day for 4 days. They received phosphate-buffered solution (35 μl; PBS), a mouse IgM isotype control (20 μg in 35 μl; ISO), or a mouse monoclonal antibody against oxidized phosphatidylcholine (20 μg in 35 μl; clone E06) intranasally each day before the first cigarette smoke exposure of the day. Bronchoalveolar lavage (BAL) cytospins were used to assess a the size of pulmonary macrophages and b were also stained with the lipophilic dye BODIPY to detect intracellular lipid accumulation in macrophages. c Black particles were counted in pulmonary macrophages and expressed as the absolute number of macrophages with no (white), 1–4 (grey) or >5 (black) intracellular particles. d Representative BAL cells cytospins of cigarette smoke-exposed and PBS or anti-OxPC (E06)-treated animals with arrows showing macrophages with internalized smoke particles. Data represent mean ± SEM; n = 4/group; representative of two independent experiments. Bars with different superscripts are significantly different from each other