Fig. 5
From: Vimentin dephosphorylation at ser-56 is regulated by type 1 protein phosphatase in smooth muscle
PP1 modulates ACh-induced actin polymerization, but not phosphorylation of c-Abl tyrosine kinase. a Tracheal rings treated with control (Ctrl) or PP1 shRNA were stimulated with 10−4 M ACh for 5 min, or left unstimulated. F/G-actin ratios in the tissues were evaluated using the actin fractionation assay. The ratios of F/G-actin under various treatments are normalized to the ratios in unstimulated tissues treated with control shRNA. Data are mean ± SE (n = 7). *, p < 0.05. b c-Abl phosphorylation at Tyr-412 in tissues treated with control or PP1 shRNA in the presence or absence of ACh was assessed by immunoblot analysis. c-Abl phosphorylation is normalized to the values in unstimulated rings treated with control shRNA. Data are mean ± SE (n = 4). N.S., not significant