Fig. 5

Airway eosinophilia in response to chronic ovalbumin (OVA) challenge and treatment with saline (sal; control), tiotropium (tio; 0.01 mM; nebulizer concentration) and/or ciclesonide (cicl; 0.01 mg/kg). Guinea pigs were treated as described in Fig. 1b. Lungs were collected 24 h after the last OVA challenge and eosinophil numbers were determined by H&E staining in the submucosa (a, i) and adventitia (b,j) of non-cartilaginous (a,b) and cartilaginous (i,j) airways. Representative images are shown in panels c-h for non-cartilaginous airways (magnification 200x) and in panels K-P for cartilaginous airways (maginification 100x). * p < 0.05, ** p < 0.01. Data represent mean ± s.e.m. of 8 animals per group