Fig. 10

IKK2 inhibition increases apoptosis of human pulmonary arterial smooth muscle cells (PASMC). Human PASMC were isolated from normal human lung following surgery and treated with an increasing concentration of the IKK2 inhibitor AS602868 (0–3 μM). Apoptosis as determined by Hoechst staining was increased from baseline in serum-starved cells (0 % FCS) at 24 h (a) and 48 h (b) (n = 3 for each group). Similar results were seen when apoptosis was measured at 24 h (c) and 48 h (d) using a DNA fragmentation ELISA (n = 5 for each group). Results represent mean ± SEM, compared using 1-way ANOVA and Bonferroni’s post-test. *p < 0.05, **p < 0.01, ***p < 0.001