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Figure 1 | Respiratory Research

Figure 1

From: Decreased expression of the NF-κB family member RelB in lung fibroblasts from Smokers with and without COPD potentiates cigarette smoke-induced COX-2 expression

Figure 1

Characterization of human lung fibroblasts. Representative immunofluorescence images of human lung fibroblasts derived from Normal (non-smoker), At Risk (Smoker without COPD) and COPD subjects. Target proteins were visualized with fluorescein (green) in combination with Hoechst (blue) nuclear stain; only the merge images are shown. C2C12 (myoblasts) and A549 (epithelial) cells were used as positive controls for desmin and keratin. Fibroblasts from each patient type were incubated with antibodies specific to vimentin (left), desmin (middle), and keratin (right). All human lung fibroblast derived by this method were positive for vimentin but negative for desmin and keratin and exhibited typical fibroblast morphology. Magnification = 20x. Representative images are shown.

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Respiratory Research

ISSN: 1465-993X

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