Figure 1
From: Regulation of IL-17A responses in human airway smooth muscle cells by Oncostatin M
HASMC responses to OSM and IL-17A. HASMC were cultured and prepared for stimulation as described in methods, where each treatment is completed in quadruplicates within each cell line. Cells were then stimulated in medium supplemented with 2% FBS and 0–50 ng/ml of the indicated gp130 cytokines. Protein concentrations in 24-hour supernatants were determined by ELISA for (A) MCP-1/CCL-2 (Left panel) and IL-6 (Right panel). *p < 0.05; ***p < 0.001 indicates statistical significance compared to no treatment as well as other indicated cytokines using One-Way ANOVA with Bonferroni’s post-test. Panel (B) (IL-6) and (C) (MCP-1) shows 24-hour supernatants from independent cell lines derived from 5 patients, prepared as above and stimulated with the indicated amounts of OSM, IL-17A or the combination as indicated. **p < 0.01 comparing indicated cytokines combinations to either cytokine alone using One-Way ANOVA with Bonferroni’s post-test.