Figure 1
From: Spinal afferent neurons projecting to the rat lung and pleura express acid sensitive channels
Tracer (DiI) distribution in thoracic tissues 6 days after application into the left pleural cavity. A) Close to the injection site, the parietal pleura (PP) is thickened and still contains an accumulation of DiI positive cells. ICM = intercostal muscle. B) The visceral pleura is more intensely labelled at the dorso-costal lung surface (arrow) than at its mediastinal surface (arrowhead). C, D) Solitary spots of DiI (arrows) are also seen at the visceral pleura of the contralateral lung (C) and the pericardium (D) while most contralateral pleural (C, arrowhead) and pericardial stretches (D, doubled arrowhead) have not accumulated tracer. LP = lung parenchyma, My = myocardium. Bars represent 100 μm in A and D, and 200 μm in B and C.