Figure 1

In PBMC, human monocytes are the major source of IL-8 production in response to CS medium stimulation. a) Different fractions of peripheral blood cells were isolated and stimulated with different concentrations of CS medium. Cytometric Beads Array assay was performed. Data represent the mean ± SD of two experiments conducted with different PBMC preparations. b). Human PBMC were left in culture medium (A) or were stimulated for 6 hours with CS medium (B) or LPS (C) in the presence of GolgiStop™. The cells were stained for surface marker CD14 and intracellular IL-8 expression. The data reflect gating on monocytes, based on forward and side scattered light signals. The result shown is a representative of two experiments conducted with different PBMC preparations that had similar results.