Figure 5

MUC4 glycoprotein expression on IL-4 stimulus. Panel (A): Confluent cells were treated with (Tr) 2.5 ng/ml of IL-4 for 8 h followed by a 16 h chase period in fresh medium without IL-4. (Ct) Untreated control cells. Plasma membrane protein (50 μg) was resolved on 4–20% SDS-PAGE gradient gels and evaluated with 1G8 anti-MUC4 monoclonal primary antibody and horse radish peroxidase conjugated goat secondary antibody. The Western blots are representative of three separate experiments. Panel (B): Commassie blue staining of the gels.