Endogenous laminin is required for human airway smooth muscle cell maturation

Respiratory Research

Table 2 Absolute ΔC_T values for laminin chain mRNA expression

*Gene product*	Laminin chain mRNA (absolute ΔC_T values)
	*Day 0*	*Day 7*
Laminin α1 chain	22.2 ± 0.5	23.5 ± 0.9
Laminin α2 chain	10.8 ± 0.9	8.8 ± 0.4
Laminin β1 chain	9.0 ± 0.5	7.5 ± 0.7
Laminin β2 chain	9.3 ± 0.3	8.8 ± 0.9
Laminin γ1 chain	8.8 ± 0.4	7.9 ± 0.6

C_T = threshold cycle/amplification cycle number. ΔC_T = difference between C_T value for laminin chain gene of interest versus matched 18s rRNA C_T value. The smaller the ΔC_T value the greater the mRNA expression. These absolute ΔC_T values were used to calculate fold differences in mRNA levels presented in Figure 5 using the comparative C_T method.

ISSN: 1465-993X