Figure 2

CCL2 mRNA expression by primary cultured AEC-II in response to proinflammatory cytokine stimulation for 24 h. Upper part of figure shows representative images of CCL2 mRNA amplificates in AEC-II derived from one of eight identical experiments. Expression of β-actin in the same samples demonstrates equal loading of lanes. Line 0 – 10 represent cells non-cultured, non-stimulated and stimulated with TNF-α, IFN-γ, or IL-1β, respectively. Line M indicates the molecular weight marker. The lower part of figure shows the results of densitometric analysis of the CCL2 mRNA expression. The mRNA-amplificates from each culture were quantitated individually. Values presented are the mean percentages of β-actin density ± SEM calculated from eight independent experiments. *P < 0.05 compared with non-cultured cells.