Figure 7

RT-PCR bands for CFTR (lanes 2 &3), α,β,γ subunits of ENaC (lanes 5,6,7 respectively), and NKCC1 (lane 9) and β-Actin (lane 10). Two independent sets of primers were used to detect CFTR. The presence of CFTR, ENaC, and NKCC1 may indicate that the epithelium has both absorptive and secretory functions. β-Actin was used as a housekeeper marker for control. Size ladders in 100 bp increments with lowest band equal to 100 bp (lanes 1,4,8).