Figure 2

Exogenous IL-4 during TCR/CD3 stimulation increased type 2 cytokine production in culture supernatants of CD8⁺T cells from atopic asthmatic subjects. CD8⁺ T cells were stimulated with anti-CD3 Abs+IL-2 in the presence or absence of IL-4 for 3 days followed by 4 days expansion with IL-2 alone- this cycle of stimulation/expansion was then repeated. Cells were then harvested and restimulated (1 × 10⁶/mL) with PMA and ionomycin. Supernatants were collected after 24 hours, and cytokines measured by ELISA. Cytokine data are median (quartiles 75) of 10 atopic asthmatic subjects. * p < 0.05