Table 1 Optimized conditions for RT-PCR

Gene

Primers

Thermocycle Condition

TNF-α

sense

5'-ACT GAA CTT CGG GGT GAT TG-3'

23 cycles; T_m: 57°C

antisense

5'-GTG GGT GAG GAG CAG GTA GT-3'

IL-1β

sense

5'-AGC TGC ACT GCA GGC TTC GAG ATG-3'

28 cycles; T_m: 68°C

antisense

5'-GAA CTG TGC AGA CTC AAA CTC CAC-3'

MCP-1

sense

5'-TAT GCA GGT CTC TGT CAC GC-3'

24 cycles; T_m: 58°C

antisense

5'-TTC CTT ATT GGG GTC AGC AC-3'

MIP-1β

sense

5'-CGT GTC TGC CTT CTC TCT CC-3'

24 cycles; T_m: 58°C

antisense

5'-CAC AGA TTT GCC TGC CTT TT-3'

ICAM-1

sense

5'-AGG TAT CCA TCC ATC CCA CA-3'

22 cycles; T_m: 58°C

antisense

5'-CTT CAG AGG CAG GAA ACA GG-3'

VCAM-1

sense

5'-CGG TCA TGG TCA AGT GTT TG-3'

24 cycles; T_m: 57°C

antisense

5'-ACC CTC ATG TAG CCT TGT GG-3'