Figure 1From: Chlamydophila pneumoniae induces expression of Toll-like Receptor 4 and release of TNF-α and MIP-2 via an NF-κB pathway in rat type II pneumocytesTLR4 mRNA expression in type II cells. Detection of TLR4 (upper lane) and TLR2 mRNA expression (middle lane) in type II cells after exposure to C. pneumoniae for 2 hours (1) and in non-infected control cells (2) by semiquantitative polymerase chain reaction. GAPDH (lower lane) served as internal control. After 2 hours of incubation with C. pneumoniae TLR4 mRNA expression in type II cells approximately doubled whereas TLR2 mRNA remained unchanged.Back to article page