Figure 1
LPS-induced IL-8 and HBD2 expression in A549 and HEK293 cells. (A) A549 or HEK293 cells (3 × 105/ml) were left untreated (control) or stimulated for 24 h with LPS (10 or 50 μg/ml), IL-1β (100 ng/ml) or TNFα (10 ng/ml). Levels of IL-8 in supernatants were measured by ELISA and values are expressed as ng/ml. Assays were performed in duplicate a minimum of three times. Values are expressed as mean+/- S.E. (n = 3). (B) Total RNA was extracted from A549 or HEK293 cells, reverse transcribed into cDNA and used as a template in PCR reactions using HBD2 gene-specific primers. Products were electrophoresed in 1.5% TBE agarose gels containing 0.5 μg/ml ethidium bromide and visualized under UV. + represents positive control PCR reaction and lanes 1–4 represent untreated cells and cells stimulated for 24 hours with LPS (10 μg/ml), IL-1β (100 ng/ml) or TNFα (10 ng/ml), respectively. Gels are representative of three independent experiments using cultures from different time points.