Table 1 Primer sequences and amplicon sizes. The primer sets work under identical PCR cycling conditions to obtain simultaneous amplification in the same run. Sequences were taken from GeneBank, Accession numbers are given.
|  | Genbank Accession | Primer Sequence (5' → 3') | Amplicon Length [bp] | |
|---|---|---|---|---|
Gene | Â | Forward | Reverse | Â |
PBGD | M28664 | GGTACAAGGCTTTCAGCATCGC | ATGTCCGGTAACGGCGGC | 135 |
Col1a1 | U08020 | CCAAGGGTAACAGCGGTGAA | CCTCGTTTTCCTTCTTCTCCG | 124 |
Col1a2 | X58251 | TGTTGGCCCATCTGGTAAAGA | CAGGGAATCCGATGTTGCC | 113 |
Col3 a1 | X52046 | TCAAGTCTGGAGTGGGAGG | TCCAGGATGTCCAGAAGAACCA | 92 |
CA3 | M27796 | GACGGGAGAAAGGCGAGTTC | CAGGCATGATGGGTCAAAGTG | 101 |
Mgp | D00613 | GTGGCGAGCTAAAGCCCAA | CGTAGCGCTCACACAGCTTG | 101 |
Myl6 | U04443 | CTTTGAGCACTTCCTGCCCA | CCTTCCTTGTCAAACACACGAA | 101 |
Spi3 | U25844 | TCCTGCCTCAAGTTCTATGAAGC | TGTTGATGTGCTGTCGGGAC | 82 |
Cytb245b | M31775 | TTTCGGCGCCTACTCTATCG | TCTGTCCACATCGCTCCATG | 101 |
Bzrp | D21207 | GAAACCCTCTTGGCATCCG | CCTCCCAGCTCTTTCCAGACT | 105 |
Psap | U27340 | GCAGTGCTGTGCAGAGATGTG | TCGCAAGGAAGGGATTTCG | 104 |
Tie2 | E08401 | GCCGAAACATCCCTCACCT | TGGATCTTGGTGCTGGTTCAT | 102 |
PDGFb | AF162784 | CGCCTGCAAGTGTGAGACAAT | CGAATGGTCACCCGAGCTT | 105 |
SRF | AB038376 | GTCTCCCTCTCGTGACAGCAG | CAGTTGTGGGTACAGACGACGT | 101 |
VEGF-R1/FLT1 | D88689 | GGAGCTTTCACCGAACTCCA | TCTCAGTCCAGGTGAACCGC | 101 |
TGF-β1 | M13177 | GCCCTGGATACCAACTATTGCTT | AGTTGGCATGGTAGCCCTTG | 127 |
FGF2 | NM_008006 | AGCGACCCACACGTCAAACT | CGTCCATCTTCCTTCATAGCAAG | 104 |
Tsp1 | J05605 | ACAGTTGCACAGAGTGTCACTGC | CATTCACCATCAGGAACTGTGG | 103 |
CD36 | L23108 | CCACTGCTTTCAAAAACTGGG | GCTGCTGTTCTTTGCCACG | 101 |
CD81 | X59047 | CCTCAGGCGGCAACATACTC | GGCTGCAATTCCAATGAGGT | 101 |
FK506bp1a | X60203 | CAAGCAGGAGGTGATCCGAG | CGGTGGCTCCATAGGCATAG | 104 |
bFGF1 precursor | X51893 | TACAAGAAAACCACCAACGGC | CCAAAAGACCACACATCGCTC | 101 |
Il-9 receptor | M84746 | GGCAGCAGCGACTATTGCAT | ACACAGGAAGGGCCACAGG | 115 |
Cyt cVIIc | X52940 | GGTTCACGACCTCCGTGGT | CATCATAGCCAGCAACCGC | 101 |
Ogn | D31951 | GACCTGGAATCTGTGCCTCCT | ACGAGTGTCATTAGCCTTGCAG | 114 |
Ptbp1 | X52101 | TGGTGTGGTCAAAGGCTTCA | GCAGTTCAATCAGCGCCTG | 101 |
S100A4 | D00208 | AGGAGCTACTGACCAGGGAGCT | TCATTGTCCCTGTTGCTGTCC | 103 |