Effects of nano particles on antigen-related airway inflammation in mice

Respiratory Research

Table 3 Protein levels of Th2 cytokines in the lung tissue supernatants.

Group	Animals	IL-5	IL-13	IL-4
	(n)	(pg/total lung tissue supernatants)
vehicle	16	5.5 ± 1.1	4.0 ± 1.1	204.3 ± 13.1
14 nm	13	4.6 ± 1.9	7.4 ± 4.3	194.7 ± 12.8
56 nm	14	7.1 ± 1.9	21.1 ± 8.9	204.3 ± 12.5
OVA	16	26.8 ± 11.1	16.2 ± 7.3	216.5 ± 14.8
OVA + 14 nm	16	113.7 ± 32.0** ^{## $}	120.3 ± 42.4** ^{## $}	178.5 ± 16.2
OVA + 56 nm	17	88.8 ± 38.0** ^{# $}	61.8 ± 27.5*	170.8 ± 17.6^#

Six groups were intratracheally inoculated with vehicle, nano particles, OVA, or the combination of OVA and nano particles for 6 wk. Lungs were removed and frozen 24 h after the last intratracheal administration. Protein levels in the lung tissue supernatants were analyzed using ELISA. Results are shown as mean ± SEM. *P < 0.05 versus vehicle, **P < 0.01 versus vehicle, ^#P < 0.05 versus OVA, ^##P < 0.01 versus OVA. ^$P < 0.05 versus nano particles. ^$P < 0.01 versus nano particles.

ISSN: 1465-993X