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Table 6 Effect of hyperoxia on the mRNA content of TNFα, TNFRI, and caspases in TIIcells

From: Inhibition of TNFalpha in vivo prevents hyperoxia-mediated activation of caspase 3 in type II cells

 

number of cycles (Δct)

increase of mRNA

 

Control

Hyperoxia

ΔΔct

-fold

TNFα

3.66 ± 0.66

0.93 ± 0.30*

-2.73

6.6

TNFRI

5.02 ± 0.19

4.44 ± 0.11*

-0.58

1.5

Caspase 8

10.3 ± 0.89

9.15 ± 0.35

-1.15

2.2

Caspase 3

5.33 ± 0.25

4.29 ± 0.48*

-1.04

2.1

  1. The mRNA of three animals per group was isolated and combined. The content of specific mRNA was determined using Real time-PCR. In parallel, GAPDH was determined in each run as internal standard. The values are given as number of GAPDH-corrected cycle (Δct) ± SD of n = 3 determinations. ΔΔct is the difference of the number of cycles between control and hyperoxia; a decrease of Δct indicates an increase of the mRNA, also given as a factor of increase. Asterisk indicates a significant difference to control (p < 0.05).
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Respiratory Research

ISSN: 1465-993X

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