Figure 2
From: Changes in the expression of NO synthase isoforms after ozone: the effects of allergen exposure
Western blot analysis of the expression of nNOS, eNOS, and iNOS in the lung tissue. Mice were categorized with OVA sensitized-saline challenged group (lane 1 and 2), OVA sensitized-ozone exposed group (lanes 3 and 4), and OVA sensitized-ozone exposed and OVA challenged group (lanes 5 and 6). Lung tissues were lysed and the extracts immunoblotted with antibody directed against nNOS, eNOS, and iNOS using a horseradish peroxidase-labeled goat antirabbit IgG (1:1,200) and enhanced chemiluminescence detection system. Each column represents the densitometric analysis. * p < 0.01 compared with OVA sensitized-saline challenged group.