Figure 4

CINC expression in lung tissue (A, C) and bronchoalveolar lavage fluid (B, D). The groups represent rats treated as follows: The groups represent rats treated as follows: Saline, saline (IT); LPS, LPS (IT); LPS-SP600125, LPS (IT) and a pretreatment with SP600125(IO), Saline-SP600125, saline (IT) and a pretreatment with SP600125(IO); LPS-PD98059, LPS (IT) and a pretreatment with PD98059 (IO), Saline-PD98059, saline (IT) and a pretreatment with PD98059 (IO). Animals were sacrificed 4 hours after LPS treatment. Western blots with anti-CINC antibodies were performed on the samples of lung tissue and BAL fluid. Densitometry of CINC bands is expressed in arbitrary densitometric units. Values are represented as means ± SEM of results from 5 rats in each group. * Significant differences between saline, p < 0.05, and + significant difference compared with LPS group, p < 0.05.