Figure 5

Percentage of intracytoplasmic cytokine-producing cells within αβ T cells (top) and γδ T cells (bottom) after 10 days of culture with cytosolic extract fromPseudomonas aeruginosa, followed by 4 hs restimulacion with PMA-Io. Intracytoplasmic cytokine expression was detected, by three color cytometry, in 10000 gated T lymphocytes. Column values correspond to mean, bar values correspond to standard error of the mean. Significant differences were not found between groups (ANOVA). p values were obtained from means comparison between αβ and γδ T lymphocytes within each group: controls (n = 17), Cystic fibrosis patients infected by PA (CFp, n = 9) and Cystic fibrosis patients not infected by PA (CFn, n = 4) (Student t test for paired samples).