Figure 6

Micro C18 RP-HPLC electrospray MS/MS analysis of tryptic peptides derived from spot 4 (Cynodon dactylon). Peptides from tryptic digestion of spot 4 (Fig 5a) were separated using micro C18 RP-HPLC and the eluate analysed by ESI-MS/MS. A. Base peak mass chromatogram, each peak is labelled with the retention time, mass, charge state and identity determined by low energy CID tandem MS of the multiply-charged precursor ions. B. MS/MS spectrum of a triple charged precursor ion m/z 741.7 corresponding to the sequence ITDKNYEHIAAYHFDLSGK from allergen Cyn d 1 was identified by database searches (Mascot).