Table 5 Genes for which differential expression was reanalysed by qRT-PCR in independent nasal cell samples
Gene symbol | Affymetrix ID | Gene description | FC (Array) | FC (QPCR) | p (t test) |
|---|---|---|---|---|---|
Upregulated in CF | |||||
ADM | 202912_at | Adrenomedullin | 2.9 | 1.7 | 0.08 |
AQP9 | 205568_at | Aquaporin 9 | 4.9 | 1.8 | 0.41 |
AREG | 205239_at | Amphiregulin | 3.6 | 1.3 | 0.21 |
GJA1 | 201667_at | Gap junction protein alpha 1 or Connexin 43 | 3.4 | 2.7 | 0.22 |
IGFBP3 | 210095_s_at | Insulin-like growth factor binding protein 3 | 2.2 | 1.2 | 0.49 |
NDRG1 | 200632_s_at | N-myc downstream regulated gene 1 | 2.6 | 2.0 | 0.18 |
TMEM45a | 219410_at | Transmembrane protein 45A or DERP7 | 3.0 | 1.0 | 0.5 |
Downregulated in CF | |||||
SCGB1A1 | 205725_at | Secretoglobin family 1A member 1 or Uteroglobin | −3.3 | −2.3 | 0.11 |
SPAG6 | 210033_s_at | Sperm associated antigen 6 | −2.5 | −3.4 | 0.1 |
TEKT1 | 239216_at | Tektin 1 | −3.3 | −3.0 | 0.12 |
reference genes | |||||
ACTB | 3 probesets | Actin beta | mean 1.2 | 1.2 | / |
GAPDH | 3 probesets | Glyceraldehyde 3-phosphate dehydrogenase | mean 1.1 | 0.8 | / |