Figure 2
From: Th17 cytokines induce pro-fibrotic cytokines release from human eosinophils
IL-17 and IL-23 enhance eosinophil expression of pro-fibrotic cytokines. (A) Surface expression of IL-17R on eosinophils (1×106 cells) isolated from healthy and asthmatics was determined by flow cytometry. Blots are representative data for eosinophils isolated from one healthy control and one asthmatic patient. The graph shows arithmetic mean ± SD of IL-17R positive eosinophils as percentage of total eosinophils (n = 5). 2×106 peripheral blood eosinophils isolated from 10 asthmatic and 10 controls subjects were stimulated with IL-17A, F, and IL-23 (50 ng/ml or 25 ng/ml) alone or in combination for 4 hrs. Total RNA was extracted and mRNA levels of TGF-β and IL-11 were then quantified using real-time PCR. mRNA expression levels of TGF-β (B) and IL-11 (C) were normalized with GAPDH for asthmatic versus healthy individuals.