Figure 1
From: Th17 cytokines induce pro-fibrotic cytokines release from human eosinophils
Basal expression of pro-fibrotic cytokines by human eosinophils isolated from asthmatic and controls subjects. Eosinophils were isolated from 10 asthmatic and 10 controls subjects and total RNA was extracted from 2×106 million cells and quantified using real-time PCR. A: Level of expression of TGF-β1 and IL-11 mRNA in eosinophils of asthmatic versus control subjects (n = 10). B: Levels of TGF-β1 and IL-11 cytokines within the supernatant of un-stimulated eosinophils (n = 10) as determined by ELISA assay. (C-D) Effect of Th1 and Th2 cytokines on asthmatic eosinophil TGF-β1 and IL-11 transcripts levels. Level of expression of TGF-β1 (C) and IL-11 (D) mRNA as quantified by real-time PCR following 4 hours exposure to mediators. Data is presented as percentage of basal expression (n = 10).