Figure 3

Pathological changes in lung and adrenal medulla tissues in offspring rats. Lung and adrenal medulla tissue sections (4 μm) from pups were stained with H&E, and the morphological changes were observed under a light microscope. Representative micrographs (100×) of lungs from OCP (A), OAP (B), ONP (C), and OANP rats (D). Representative micrographs (100×) of adrenal medulla in OCP (E), OAP (F), ONP (G), and OANP rats (H). Ultrathin sections (50 nm) from adrenal medullae were prepared from the different specimens and stained with uranyl acetate and lead citrate. Examinations of ultrastructural changes were carried out with an H-7500 TEM. Representative electron micrographs (10,000×) of adrenal medullae in OCP (I), OAP (J), ONP (K), and OANP rats (L). Colored arrows indicate chromaffin granules (red), swollen cytoplasm (blue), neurite-like processes (yellow), and deposition of collagen tissue (black).