Figure 4

Fizz1 overexpression in the lung has no effect on bleomycin-induced inflammation. (A) CCSP/- mice lungs were exposed to saline or bleomycin via the I.T. route. Fizz1 transcripts were measured using RT-PCR at day10 post-bleomycin. Statistical significance is measured using unpaired student’s t test (n = 5/group). (B) Schematic representation of Fizz1 induction by feeding doxycycline food and administration of 0.15U of bleomycin via the I.T. route. (C) CCSP/Fizz1 mice exposed to bleomcyin demonstrate accelerated weight loss compared with CCSP/- controls receiving bleomycin. Data shown are mean ± SEM (n = 7-8/group). (D) Total BALF cells quantified at day10 post bleomycin or saline. Data shown are mean ± SEM (n = 7-8/group). Statistical significance is measured using unpaired student’s t test. (E) Absolute counts of macropahges in BALF cells were obtained from cell differentials. Data shown are mean ± SEM (n = 7-8/group). Data represent one of two independent experiments showing similar results.