Figure 3

BAL mDCs from sarcoidosis patients are immunocompetent in co-cultures with T cells. Induction of T cell proliferation and differentiation by mDCs isolated from BAL from sarcoidosis patients. mDCs (defined as low autofluorescent, lineage mix negative, HLA-DR⁺, CD11c⁺ cells), isolated from BAL from sarcoidosis patients and healthy controls, were tested in a MLR with naïve allogeneic CD4⁺ T cells. (A) After 5 days of co-culture, T cell proliferation was measured using 3H-thymidine incorporation. (B) T cell differentiation was assessed using markers for Tregs (CD25+ Foxp3⁺ CD127 ^low, memory T cells (CD45RO⁺), Th1 (CXCR3⁺) and Th2 (CCR4⁺) cells. (C) Cytokines were measured in the supernatant of the co-culture using a bead-based cytokine kit. Data are presented as boxplots, whiskers indicate minimum and maximum of the data.