Figure 1
From: Evidence for local dendritic cell activation in pulmonary sarcoidosis
Gating and sorting strategy for mDCs and pDCs. Dead cells were excluded from the total fraction of the BAL cells using DAPI as a dead-live marker (not shown). The alive, low auto fluorescent, lineage mix (CD3-, CD14-, CD16-, CD19-, CD56-) FITC negative fraction was expressed in the Pe-Cy7 channel for HLA-DR expression. HLA-DR positive cells were subsequently expressed in the PE-Cy5 channel for CD11c expression. Cells positive for CD11c are mDCs. CD11c negative cells were expressed in APC for CD123: cells positive for CD123 are pDCs.