Table 1 Experimental design and numbers of animals per group
| Â | Control + RA (WT) | Control + O2 (WT) | VT 30 ml/kg + RA (WT) | VT 30 ml/kg + O2 (WT) | VT 30 ml/kg + O2 with enoxaparin (WT) | VT 30 ml/kg + O2 (Akt+/-) |
|---|---|---|---|---|---|---|
HMGB1, cell isolation (8 hours of ventilation) | 5 | 5 | 5 | 5 | 5 | 5 |
Phospho-Akt, HMGB1 mRNA (2, 4, 8 hours of ventilation) | 5 | 5 | 5 × 3 | 5 × 3 | 5 | 5 |
MPO, lung edema (8 hours of ventilation) | 5 | 5 | 5 | 5 | 5 | 5 |
EBD assay (8 hours of ventilation) | 5 | 5 | 5 | 5 | 5 | 5 |
MDA/GSH, IHC (8 hours of ventilation) | 5 | 5 | 5 | 5 | 5 | 5 |