Figure 7
From: Therapeutic efficacy of TBC3711 in monocrotaline-induced pulmonary hypertension
Effect of TBC3711 on collagen content and cardiomyocyte size in right ventricles of monocrotaline (MCT)-injected rats. Right ventricle (RV) sections were stained with 0.1% Sirius Red in picric acid to assess the collagen content and also with fluorescein isothiocyanate conjugated wheat germ agglutinin/diamidino phenylindole to assess the cardiomyocyte size. Photomicrographs were quantified to determine the interstitial collagen fraction and cardiomyocyte size by using computer-assisted image analysis software. (a, b) Representative photomicrographs are presented (A - healthy control, B - MCT-placebo and C - TBC3711 treated group; arrows indicate collagen formation - red color). (c) Collagen area and (d) cross sectional area are shown for different experimental groups. Bars represent mean ± SEM. One-way ANOVA with Newman-Keuls multiple comparison post-hoc test was performed for statistical analysis. ***P < 0.001.