Figure 3

Effect of adrenomedullin (AM) on adhesion molecules expression and MPOactivity. Immunohistochemical analysis of lung sections obtained from BLM-treated mice revealed a positive staining for ICAM-1 (B) and P-selectin (F) in the injured tissues, mainly localized around the vessels. In BLM mice treated with AM, the staining for ICAM-1 (C) and P-selectin (G) was significantly reduced when compared with BLM group No positive staining for ICAM-1 and P-selectin was observed in lung tissues obtained from sham-operated mice (A and E, respectively). Densitometry analysis of immunocytochemistry photographs (n = 5 photos from each sample collected from all mice in each experimental group) for ICAM-1 and P-selectin from lung tissues was assessed (H). The assay was carried out by using Optilab Graftek software on a Macintosh personal computer (CPU G3-266). Data are expressed as % of total tissue area. MPO activity was increased by BLM-administration, when compared with lung tissues from sham animals (C). In contrast, a decrease in this enzyme activity was observed in mice treated with AM (C). The figure is representative of at least 3 experiments performed on different experimental days. Data are expressed as mean ± standard deviation from n = 10 mice for each group. *P < 0.01 vs sham, °P < 0.01 vs bleomycin + vehicle.