Figure 4From: Detrimental effects of albuterol on airway responsiveness requires airway inflammation and is independent of β-receptor affinity in murine models of asthmaEffect of albuterol on AHR in allergic mice. Respiratory mechanics time course following methacholine challenge. Parameters from fitting the constant phase model to input impedance data; R n is Newtonian resistance of the conducting airways; H is lung elastance and G is tissue resistance. Left column: Allergic Balb/C mice; AHR measured as dose-response time-course to increasing doses of methacholine inhalation in OVA sensitized Balb/C mice. Animals were treated with (RS)-, (R)- or (S)- albuterol (n = 11, 12 and 10) twice daily for seven days with the last administration 18 hours before experiment. PBS (n = 10) was used as vehicle control. H was significantly elevated over PBS control at the 12.5 and 50 mg/ml doses of methacholine by (RS)-, (R)- and (S)- albuterol. * p < 0.05, ** p < 0.01 and *** p < 0.001. Right column: Naïve Balb/C mice; AHR assessment in naïve Balb/C mice. Animals were treated with (RS)-, (R)-, (S)- albuterol or control PBS, n = 8 per group.Back to article page