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Table 2 Antibodies Used

From: Nasal lavage natural killer cell function is suppressed in smokers after live attenuated influenza virus

Antibody [Reference]

Isotype Control

Dilution

Company

Clone

Catalog Number

Anti-human CD56 [48]

Mouse IgG2b, κ

1:100

RnD Systems

301021

MAB24081

Anti-human perforin [49]

Mouse IgG2b, κ

1:100

BDBiosciences

δG9

556434

APC anti-human NKG2D [50]

Mouse IgG1, κ

1:10

Biolegend

1D11

320808

APC-Cy7 anti-human CD45 [51]

Mouse IgG1, κ

1:20

BDBiosciences

2D1

557833

FITC anti-human CD16 [52]

Mouse IgG1, κ

1:10

Beckman Coulter

3G8

IM0814U

Pacific Blue anti-human CD14 [53]

Mouse IgG2a, κ

1:20

BDBiosciences

M5E2

558121

PE anti-human CD56 [54]

Mouse IgG1, κ

1:10

BDBiosciences

B159

555516

PE-Cy5 anti-human CD4 [55]

Mouse IgG1, κ

1:10

BDBiosciences

RPA-T4

555348

PerCP anti-human CD3 [56]

Mouse IgG1, κ

1:10

BDBiosciences

SK7

347344

PerCP/Cy5.5 anti-human CXCR3[57]

Mouse IgG1, κ

1:40

Biolegend

TG1/CXCR3

334905

Biotinylated horse anti-

mouse IgG [58]

Horse

1:200

Vector Labs

 

BA-2000

APC mouse IgG1, κ

 

1:100

BDBiosciences

 

555751

FITC mouse IgG1, κ

 

1:100

BDBiosciences

 

555748

Pacific Blue mouse IgG2a, κ

 

1:100

BDBiosciences

 

558118

PE mouse IgG1, κ

 

1:100

ebioscience

 

12-4714-81

PE-Cy5 mouse IgG1, κ

 

1:100

BDBiosciences

 

555750

PerCP mouse IgG1, κ

 

1:100

BDBiosciences

 

550672