Figure 5

Role for CAT2 in macrophage arginase activity. In A, peritoneal macrophages were isolated from CAT2-deficient (CAT2KO) and wild type mice and arginase activity was measured by hydrolysis of extracellularly added ¹⁴C-arginine by intact macrophages. A representative experiment (out of 5 total; 3 with mice on C57Bl/6 and 2 on FVB/N background) is shown. Data are mean ± standard deviation of triplicate measurements. In (B), macrophages from wild type and CAT2-deficient mice had arginase activity measured following cell lysis by detergent.