Figure 4

Bleomycin treatment of CAT2-deficient mice. Wild type and CAT2-deficient mice (C57Bl/6 background) were challenged with bleomycin (bleo) i.t. and BALF cells (A) and hydroxyproline levels (B) were measured 14 days later. Representative of 3 experiments is shown. Data are mean ± standard deviation of 5-6 mice/group. In (C), representative photomicrographs of 3 individual mice stained with trichrome staining are shown (WT-wild type; KO- CAT2-deficient mice). In D and E, quantification of trichrome staining in bleomycin-treated mice in a representative (D) and four individual experiments (E) is shown (one using mice on C57Bl/6 background and 3 on FVB/N background). Lungs were scored on a scale from 0-3 for intensity and extent of involvement. (D) P values by Kruskal-Wallis with Dunn's Multiple Comparison Test. (E) n = 4-8 mice/group (only bleomycin-treated groups are shown) in each experiment with a total of 19 wild type and 21 CAT2-deficient mice. P values shown are by Mann-Whitney test. 2-way ANOVA (variables: genotype and experiment) identified genotype as the statistically significant (P < 0.0001) source of variation (interaction and experiment P = not significant).