Figure 4

COX mRNA levels in lung tissues after LPS stimulation. Twenty-four hours after LPS instillation, mice were sacrificed and total RNA was extracted from homogenates of perfused lung. COX1 (A) and COX2 (B) mRNA levels were determined by quantitative PCR and expressed as RQ. RQ: relative quantity normalized to HPRT mRNA; * p < 0.05 LPS-treated vs saline-treated mice. ns, no significant differences between LPS-treated CFTR -/- vs CFTR +/+ mice.