Figure 6

Validation of the 2-DE proteomic data by western blot analysis. (A) A representative western blot visualizing the ERp29, RhoGDI2 and S100A8 expression levels. β-actin was used to demonstrate equal loading. NC = normal control group (n = 15) and AS = asthmatic group (n = 14). (B) The densitometric quantification of individual protein is expressed as the fold change compared to β-actin. The data are presented as the mean ± SD of triplicate experiments, and similar results were seen in all experiments. Statistical comparisons were made using Student's t-test. *p < 0.05, **p < 0.01 comparing the asthmatic group with the control group. The expression pattern of these proteins is in agreement with the 2-DE results.